Allogeneic hematopoietic stem cell transplantation is widely used in malignant diseases, blood system diseases and hematopoietic failure immunodeficiency disease and achieved remarkable results, but the graft rejection, relapse after transplantation, transplantation related complications such as graft-versus-host disease (graft versus host disease, GVHD) is a life-threatening. The main problem affecting the success rate of transplantation, if can and clinical intervention in early monitoring, can effectively prevent the occurrence of graft failure and GVHD. Therefore, it is necessary to identify the implantation status of transplant recipients and provide a reliable basis for clinical intervention.

Short tandem repeat (short tandem repeat, STR), also known as micro satellite DNA (microsatellite DNA), is composed of 2-6 base sequence as the core unit, a class of DNA tandem repeat sequence formation, because the core unit repeats between individuals is highly rich diversity and quantity, constitute the STR polymorphism. It has become a powerful tool for the identification of different individuals because it provides a large number of genetic markers. At present, STR technology has become a gold standard recommended by the bone marrow transplant registry (IBMTR) for the detection of recipient chimerism after transplantation.

Product introduction

In order to better observe the chimeric state in vivo and to evaluate the efficacy of transplantation, we will use STR method to guide the clinical treatment and prevent the recurrence of the disease.

Sampling requirements

1. for the first time, 3 samples are required: the patient's transplant, the patient's transplant and the donor's sample.

2. pre transplant samples can be used to collect blood before transplantation, such as without backup, to collect hair with hair follicles and buccal buccal swabs instead.

3. do not use heparin anticoagulant tube.

Sample preservation conditions

1. anticoagulant temperature was transported to the laboratory within 3 days or within 7 days of 2--8 C.

2. hair or buccal membrane wiping cotton sticks are placed independently in plastic bags.

3. in the case of rain and rain, please put the desiccant in the package to prevent mildew.

Clinical significance

After 1. allogeneic hematopoietic stem cell transplantation, the chimeric state is monitored to assist the doctors in clinical medication.

After 2. allogeneic hematopoietic stem cell transplantation, donor stem cells were implanted in the evidence.

3. the recipients of allogeneic stem cell transplantation who have been discharged from the hospital can monitor the survival rate of the transplanted stem cells.